| 日時 | 令和7年5月9日（金）17：00〜 |
| 場所 | 動物生命科学研究センター5階 セミナー室 |
| 演題 | 「The challenges of pluripotent stem cell-based germline chimeras in rabbits」 |
| 講師 | Pierre Savatier 先生 （ Stem-Cell and Brain Research Institute, INSERM ） |
〈 演題要旨 〉
Pluripotent stem cells have long been used to produce knockout mice via germline chimera technology. However, aside from the rat, this approach has not been successfully applied to other mammals. I will describe our long-term efforts to generate rabbit embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) lines. Building on our investigation of the pluripotency continuum in rabbit embryos, I will demonstrate that rabbit iPSCs can be reprogrammed using KLF2, ERAS and PRMT6, which enables them to efficiently colonize host embryos. These chimeric embryos develop into fetuses and newborn rabbits, with iPSCs contributing up to 100 % to certain organs. Notably, female rabbits generated via this method are healthy and transmit the iPSC genome to their offspring, confirming germline chimerism. This advancement lays the groundwork for developing rabbit models of human disease and, when extended to non-human primates, could enable the generation of monkey models that capture complex genetic traits beyond the scope of conventional CRISPR-based embryo editing.
Reference:
H.-T. Pham, F. Perold, Y. Pijoff, N. Doerflinger, S. Rival-Gervier, M. Givelet, A. Moulin, M. Ressaire, E. Da Silva Fernandes, L. Jouneau, V. Duranthon, F. Wianny, B. Pain, I. Plotton, T. Joly, M. *Afanassieff, P. *Savatier, N. *Beaujean (2025). Efficient Generation of Germline Chimeras in a Non-Rodent Species Using Rabbit Induced Pluripotent Stem Cells. Nat. Commun. In press.